Part:BBa_K2294007:Design

Synthtic minimal Galactose induced promoter + Kozak sequence

Short synthetic Galactose induced promoter.

In Redden 2015 the to this date shortes fungal promoters were created. Short core elements were combined with a TATA box, Transcription Start SIgnal (TSS) and a neutral spacer. For enabling galactose inducibility the core promoter was combined with minimal galactose inducible UAS (Upstream activating element). In addition a Kozak sequence was fused to the promoter.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

a BpiI recognition site in the Gal1 UAS was removed

Source

Sequence taken from "The development and characterization of synthetic minimal yeast Promoters" Redden 2015

Promoter was synthesized as gBLOCK® Gene Fragment by IDT

References

Redden, H., & Alper, H. S. (2015). The development and characterization of synthetic minimal yeast promoters. Nature Communications, 6, 7810. https://doi.org/10.1038/ncomms8810